Utilization of membranous lipid substrates by membranous enzymes. Hydrolysis of sphingomyelin in erythrocyte ‘ghosts’ and liposomes by the membranous sphingomyelinase of chicken erythrocyte ‘ghosts’
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چکیده
منابع مشابه
Interaction of membranous enzymes with membranous lipid substrates. Hydrolysis of diacylglycerol by lipase in rat brain microsomes.
The phospholipids in rat brain microsomes were labeled with tritium by intracerebral administration of radioactive fatty acids and converted to diacylglycerol with phospholipase C. The latter lipid was hydrolyzed in situ at pH 4.8, to monoacylglycerol and fatty acid by the endogenous microsomal lipase. This paper provides an experimental approach to determine whether the lipid was degraded by e...
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this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
Permeability Properties of Erythrocyte Ghosts
1. Erythrocyte ghosts from human blood were produced by gentle water hemolysis. The ghost-containing hemolysate (about 20 mN) was added to media of different composition (KCl, NaCl, glucose, sucrose, etc.) and varying concentration ranging from 8 to 840 mN. The volume changes of the ghost cells were followed by a light absorption method. The potassium and sodium concentrations were also analyze...
متن کاملDielectric dispersion of erythrocyte ghosts.
The underlying mechanism of the low-frequency dielectric dispersion, called the alpha dispersion, of osmotically lysed erythrocytes (erythrocyte ghosts) has remained open since its finding [H. P. Schwan and E. L. Carstensen, Science 125, 985 (1957)]. The alpha dispersion is peculiar to erythrocyte ghosts and has never been observed for intact erythrocytes. Numerical calculation based on interfa...
متن کاملA Basis of the Crenation of Erythrocyte Ghosts by Electrolytes
Erythrocyte ghosts isolated in hemolytic hypotonic buffers, pH 7.0-8.0, 0-4C were previously observed to be crenated by electrolytes. This shape transformation, which occurred rapidly and reversibly, was attributed to electrostatic interactions of cations with flexible filamentous anionic spectrin, the major protein component of the skeleton and/or anionic phospholipids, since divalent cationic...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1980
ISSN: 0264-6021
DOI: 10.1042/bj1870115